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KMID : 0613820090190091232
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Journal of Life Science
2009 Volume.19 No. 9 p.1232 ~ p.1238
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Rim1α and Neurexin3 Regulate Synaptosomal Dopamine Release
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Lee Sang-Kyung
Shim Joo-Cheol
Kim Young-Hoon
Kong Bo-Geum
Seog Dae-Hyun
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Abstract
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Neurotransmitter release is regulated by various proteins of the active zone in the presynaptic nerve terminals. Dopamine (DA) is an essential neurotransmitter associated with the pathophysiology of diverse behavioral and mental illness such as schizophrenia and drug addiction. We measured synaptosomal DA release of knockout (KO) mice which lacked major genes related to neurotransmitter release. Synaptosomal DA uptake and release were performed and measured using [³H]-DA and superfusion experiments. 3 of the 17 KO mice exhibited altered DA release compared to their littermate controls. In Rim1α KO, [³H]-DA release evoked by membrane depolarization significantly decreased. Both basal (physiological buffer-evoked) and membrane depolarization-evoked DA release significantly decreased in dopaminergic conditional KO of Rim1αβ. Dopaminergic conditional KO of neurexin3 demonstrated a significant increase of membrane depolarization-evoked DA release. These data explain the similarities and distinctions between DA and other classical neurotransmitters such as glutamate and GABA (γ-aminobutyric acid) release. In conclusion, Rim1α and neurexin3 may be important regulators of presynaptic DA release and related to disorders of the nervous system.
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KEYWORD
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Dopamine release, neurotransmitter, Rim1α, neurexin3
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